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KMID : 0378019730160040059
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New Medical Journal
1973 Volume.16 No. 4 p.59 ~ p.69
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Effect of Reserpine on Mucus Secreting Cells in Gastrointestinal Mucosa of Rats
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Abstract
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This experiment was performed in order to study the effect of reserpine, which is one of the powerful releaser of 5-hydroxytryptamine, on mucus-secreting cells in the gastrointestinal mucosa.
Healthy adult male rats weighing about 200 g each were used as experimental animals and were divided into normal and reserpine-treated groups. Reserpine was intraperitoneally injected in a single dose of 5 mg/kg body weight to each animal and the reserpine-treated rats were sacrificed on the 1st, 3rd, 6th, 12th hour and 1st, 2nd, 5th, 7th, 10th, 14th, 21st, 28th and 40th day after the injection.
Gastrointestinal mucosal specimens from the body of the stomach, the upper part of the duodenum, and the middle part of the colon were fixed in 10% neutral formalin solution, embedded in paraffin wax, sectioned at a thickness of 6p, stained with periodic acid-Schiff reaction-hematoxylin, alcian blue pH 2.5, and alcian blue pH 1.0. Morphological and histochemical changes of gastrointestinal mucus-secreting cells were observed.
The results were as follows:
1. Mucus-secreting cells sensitively showed the initial hypersecretion following the reserpine inje￢ction, and the decrease of their mucous contents afterwards. Restoration of the mucus-secretings cells was slow and reached to nearly normal state in 40 days after the injection.
2. Strongly acidic sulfated mucosubstance and weakly acidic nonsulfated mucosubstance were sensi￢tively affected by the reserpine. but neutral mucosubstance showed less prominent changes `during the experiment.
3. The mucus-secreting cells in the gastric mucosa revealed most sensitive response to the reserpine and those in the colonic mucosa showed relatively slight changes..
4. A possible positive relationship between the abo°, e findings of mucus-secreting cells and the changes of enterocliromaffin cells under the influence of reserpine-injection was discussed.
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